144 0001785287 XXXXXXXX LIVE 0001703647 Frequency Therapeutics, Inc. 001-39062 75 HAYDEN AVENUE SUITE 300 LEXINGTON MA 02421 781-315-4600 Mitrano Richard J. Officer Common Fidelity Brokerage Services LLC

900 Salem Street Smithfield RI 02917

237 125.66 36520750 08/21/2023 NASDAQ Common 08/18/2023 Restricted Stock Vesting Issuer N 237 08/18/2023 Compensation N Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/06/2023 224 78.40 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/07/2023 220 78.25 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/10/2023 220 81.80 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/11/2023 228 84.45 Richard J. Mitrano

75 Hayden Avenue, Suite 300 Lexington MA 02421

Common 07/12/2023 220 87.45 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/13/2023 227 87.64 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/14/2023 148 89.16 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/17/2023 244 159.16 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/18/2023 249 139.44 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/19/2023 228 143.66 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/20/2023 229 146.29 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/21/2023 223 136.25 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/24/2023 234 130.53 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/25/2023 234 116.72 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/26/2023 222 113.44 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/27/2023 227 121.33 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/31/2023 222 112.02 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 07/28/2023 223 104.63 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/01/2023 238 114.00 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/02/2023 231 112.70 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/03/2023 232 130.94 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/04/2023 220 146.94 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/07/2023 235 161.82 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/08/2023 241 162.02 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/09/2023 238 157.20 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/10/2023 247 159.14 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/11/2023 240 153.48 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/14/2023 238 152.58 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/15/2023 228 148.47 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/16/2023 234 156.90 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/17/2023 230 142.88 Richard J. Mitrano

75 Hayden Avenue Suite 300 Lexington MA 02421

Common 08/18/2023 227 143.21 08/21/2023 /s/ Daniel Tucci, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Richard J. Mitrano